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Microbial Identifications

Nelson Labs offers competitive turnaround time and pricing on microbial identification services.  We specialize in accurately identifying unknown bacterial and fungal isolates from a wide range of sources.  Microbial identification tests are generally performed following bioburden tests, environmental monitoring, tissue testing, and sterility/BI testing.

Test systems available are:

  • Gram Stain: Assesses colony morphology, cell morphology, and Gram reaction to confirm culture purity and distinguish bacteria from yeast (does not provide species-level identification).
  • Genotypic Identification (DNA Sequencing): Provides species-level ID; targets the 16S rRNA gene (bacteria) and the LSU D2 gene (fungi) and then compares sequences against validated databases for precise results.
  • MALDI-TOF Identification (Matrix-Assisted Laser Desorption Ionization – Time of Flight): Uses mass spectrometry to analyze ionized proteins from bacterial or fungal samples. The resulting spectra are compared to a database for genus or species-level identification.
    • Additional information on the IDT200 identification method can be found here. Please note that MALDI-TOF identification is only available at the Salt Lake City facility.

 

Diverse Sample Acceptance: We’re equipped to handle a variety of sample types, including:

  • Isolated cultures (on plates, slopes, or in broth)
  • Mixed cultures
  • Environmental monitoring
  • Water samples
  • Contaminated product samples

Applicable Standards

  • USP <71>
  • ISO 11737
  • USP <1113>
  • USP <61>
  • USP <62>

 

Testing Locations

  • Salt Lake City, UT, USA
  • Itasca, IL, USA

Learn more about our locations and their certifications.

If you have additional questions about Microbial Identification test services, or would like to consult with the experts at Nelson Labs, just send us a request or call us at +1 (801) 290-7500.