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Organism Identification

Organism Identification

The Organism Identification tests are used to characterize product bioburden and/or environmental bioburden and to identify organisms in sterility positives and confirm the presence or absence of possible objectionable organisms in tissue.
Nelson Laboratories combines different methods that complement each other in order to provide our customers with the best options for organism identification.

View our Rapid Microbial Identifications options.

Applicable Standards

USP 71    |    ISO 11737    |    USP 1113    |    USP 61/62

Pricing/Quote

Test Price Request Quote
IDG105 - Organism ID: Genotypic identification, MicroSeq w/ Gram stain
Per sample1 - 10
11 - 19
20+
$165.00
$145.00
Quote
Add
IDG215 - Organism ID - Standard: Genotypic, MicroSeq w/ Gram stain (pure culture)
Per sample1+$85.00
Add
IDS210 - Organism ID: Classic morphological to genus
Per sample1+$175.00
Add
IDS220 - Organism ID: Classic morphological to genus with picture, per isolate
Per sample1+$200.00
Add
IDS240 - Organism ID: Biochemical confirmation
Per sample1+$40.00
Add
IDS330 - Organism ID: Vitek 2 Compact - Standard
Per sample1 - 10
11+
$140.00
$130.00
Add
IDS510 - Organism ID: Gram Stain only
Per sample1 - 5
6+
$35.00
$25.00
Add
IDS530 - Organism ID: Correlation, per set (up to 10 plates)
Per sample1+$55.00
Add
IDS720 - Organism ID: Preparation and culturing (mixed org.)
Per sample1+$30.00
Add
IDS750 - Organism Identification: Second try - alternate system, per isolate
Per sample1+$80.00
Add
IDG105 - Organism ID: Genotypic identification, MicroSeq w/ Gram stain
Per sample1 - 10
11 - 19
20+
$165.00
$145.00
Quote
IDG215 - Organism ID - Standard: Genotypic, MicroSeq w/ Gram stain (pure culture)
Per sample1+$85.00
IDS210 - Organism ID: Classic morphological to genus
Per sample1+$175.00
IDS220 - Organism ID: Classic morphological to genus with picture, per isolate
Per sample1+$200.00
IDS240 - Organism ID: Biochemical confirmation
Per sample1+$40.00
IDS330 - Organism ID: Vitek 2 Compact - Standard
Per sample1 - 10
11+
$140.00
$130.00
IDS510 - Organism ID: Gram Stain only
Per sample1 - 5
6+
$35.00
$25.00
IDS530 - Organism ID: Correlation, per set (up to 10 plates)
Per sample1+$55.00
IDS720 - Organism ID: Preparation and culturing (mixed org.)
Per sample1+$30.00
IDS750 - Organism Identification: Second try - alternate system, per isolate
Per sample1+$80.00

Turn Around Time

Standard turn around times (TAT) are listed below. Ask an expert for a specific consultation on your product.

Code Test TAT
IDG105 Organism ID: Genotypic identification, MicroSeq w/ Gram stain 7
IDG215 Organism ID - Standard: Genotypic, MicroSeq w/ Gram stain (pure culture) 7
IDS210 Organism ID: Classic morphological to genus 16
IDS220 Organism ID: Classic morphological to genus with picture, per isolate 16
IDS240 Organism ID: Biochemical confirmation 18
IDS330 Organism ID: Vitek 2 Compact - Standard 7
IDS510 Organism ID: Gram Stain only 5
IDS530 Organism ID: Correlation, per set (up to 10 plates) 7
Code TAT
IDG105 7
IDG215 7
IDS210 16
IDS220 16
IDS240 18
IDS330 7
IDS510 5
IDS530 7

Sample Specifications

IDG105: Per isolate
IDG215: sample to be submitted as a pure isolated culture on an agar plate

Study Outline

The Organism Identification tests are performed following bioburden, environmental monitoring, tissue testing, microbial limits and on sterility or BI positives.
 
Test systems available are:
  • MicroSeq® library and an Applied Biosystems/Hitachi 3500xL Genetic Analyzer
  • Gram stain
  • Vitek® 2 Compact
  • Classical Mold Identification (Genus only)
Gram stain: Isolated colonies are examined for colony morphology, Gram reaction and cell morphology.

Vitek: The microorganism is streaked onto the appropriate medium and incubated for 18 – 70 hours. It is then suspended in 3 ml of saline and tested using the appropriate card type. Identification is based on patterns of biochemical utilization in a 64-well card and through the introduction of Advanced Colorimetric™ technology.  A Gram stain is included as part of the test, so that the appropriate card type is chosen.

Classical Mold Identification (Genus only): A lactophenol cotton blue mount is used to visualize the morphological structures of the mold. The identifications are made based on morphological similarities to known organisms.

Genetic identification: This uses DNA instead of traditional biochemical reactions. It entails collecting the DNA from a microorganism (16S ribosomal DNA for bacteria and D2 region ribosomal DNA for fungi), creating many copies of the target region, then sequencing and comparing the unknown sequence to the MicroSeq library. If a match is not found in the MicroSeq library, a public database may be searched.

A Gram stain is done along with the genetic identification when testing involves sterility or BI positives and other liquid preparations. 

The Gram stain is done to verify pure culture.   It provides a phenotypic component which makes the identification more complete. 

Gram stains are recommended to determine if organisms are bacteria or yeast.