Zone of Inhibition Test
The Zone of Inhibition test is a quick qualitative method for determining activity of diffusible antimicrobial agents. It is performed on any product that contains a leachable antimicrobial chemistry, and may include solutions or treated materials. This test allows the customer to quickly and easily determine if a product has antimicrobial properties.
The test is an adaptation of the disk diffusion (Kirby-Bauer) method for antibiotic susceptibility testing. Nelson Laboratories offers quick turnaround time and quality procedures performed by an experienced and well-trained staff.
Ask an expert for a specific consultation on your product. If you are ready to submit your samples for testing, click here to fill out the Sample Submission Form.
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ZHT110 - Zone of Inhibition: Test set up and protocol
ZHT110: Quantity = Number of oranisms used
Study OutlineIn the Zone of Inhibition procedure, the test organism is spread evenly over the entire surface of an agar plate. The antimicrobial test specimen is then applied to the center of the plate.
After incubation, a “zone of inhibition,” or clear area of uninterrupted growth underneath and around the test material, indicates the presence of antimicrobial activity. An untreated control sample is tested under the same conditions to ensure adequate control of the test system.