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Resistance to Wet Bacterial Penetration

Resistance to Wet Bacterial Penetration

The Resistance to Wet Bacterial Penetration test (ISO 22610) evaluates the bacterial penetration resistance characteristics of personal protective equipment such as surgical gowns, drapes and clean air suits. This test is required by the EN 13795 guidance, which determines performance requirements and performance levels for gowns and drapes. The test is also important when seeking a CE mark for products marketed in Europe. Nelson Laboratories personnel are experts in barrier testing and we offer a one-stop shop for barrier tests required for marketing gowns and drapes in both the U.S. and Europe.

Applicable Standards

  • ISO 22610
  • EN 13795

Test Options

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HPT210 Resistance to Wet Bacterial Penetration, per product (set of 5) Add
HPT210 - Resistance to Wet Bacterial Penetration, per product (set of 5)

Study Outline

In the Resistance to Wet Bacterial Penetration test, specimens are prepared by aseptically cutting 25 x 25 cm samples from the barrier to be tested. A donor material is prepared by inoculating a polyurethane film with ~1 x 104 colony forming units (CFU) of Staphylococcus aureus bacteria. The donor material is placed on top of the test specimen with the bacteria touching the sample.

The combination of test sample and donor material is then sandwiched between a rotating petri dish and an oscillating stainless steel finger. As the sample and petri dish rotate, the finger attempts to force the bacteria from the donor material through the sample and onto the petri dish. The sample is tested for one hour with the petri dish being replaced with a fresh dish every 15 minutes.

The petri dishes are then placed in an incubator. After incubation, the number of bacteria colonies that have passed through the barrier are counted. The colony counts are used to calculate the barrier efficiency of the product.

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