The growth promotion test is used to determine the ability of agar media to support growth of an array of microorganisms. This test is performed on agar media for a variety of uses, such as media for environmental monitoring. The test can be performed quantitatively (organism counts) or qualitatively (observable growth/no growth). A pH verification of the media is also performed.
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|MIC210||Growth Promotion: Agar Media (RODAC or Agar Plate)||Add|
|MIC220||Growth Promotion: Agar Media (Airstrips or Hygicult)||Add|
|MIC230||Growth Promotion: Sterility Media, USP per lot or set||Add|
|MIC235||Growth Promotion: Agar Media, add. replicates||Add|
MIC210 - Growth Promotion: Agar Media (RODAC or Agar Plate)
MIC220 - Growth Promotion: Agar Media (Airstrips or Hygicult)
MIC230 - Growth Promotion: Sterility Media, USP per lot or set
MIC235 - Growth Promotion: Agar Media, add. replicates
MIC210: please contact the study director
MIC230: 8 separate vials/containers per lot
Study OutlineQuantitative growth promotion testing: This testing is performed by inoculating the agar media with Not More Than 100 colony-forming units (CFU) of a known culture. The challenge organisms are determined by the purpose of the media being analyzed. Each organism is plated on both the test media and a control plate (provided by Nelson laboratories). The agar is then incubated at 30-35°C for 2-3 days for aerobic bacteria or 20-20°C for 5-7 days for fungal organisms. For USP <1227> proper recovery is demonstrated by exhibiting a minimum of 70% recovery in comparison to the control plate. For USP <61> proper recovery can also be demonstrated if the recovery from the challenged test article is no greater than a two-fold difference from the organism control plate.
Qualitative growth promotion testing: This testing is performed by inoculating the agar with a turbid culture of known organism. Inoculation is accomplished by either streaking the agar with the organism or by using the stabbing method. Incubation is performed at 30-35°C for 2-3 days, or alternate incubation parameters may be accommodated as requested. The organism or panel of organisms used for inoculation can be modified to meet the requirements for the specific media. Recovery is demonstrated by characteristic growth of the inoculated organisms.
pH Testing: A pH verification of the media is also performed. This test requires one additional test article plate.