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The FTIR test is a tool to characterize and record the spectra of known materials, to provide a basis for comparison to future samples to the known material, and to help identify unknown compounds. This technique is used to compare material from lot to lot, to identify extracted material from a finished product, to identify a contaminant on a product and to verify cleaning processes. Materials tested include polymers, finished products, oil, lubricants and other organic material.

Testing is performed according to criteria outlined in the USP General Chapter 32 and the National Formulary 27, 2009, Chapter 851 on Spectrophotometry and Light Scattering and Chapter 198 on Spectrophotometric Identification tests. Nelson Laboratories offers fast, accurate and dependable FTIR testing services.

Applicable Standards

  • ISO 10993-12
  • ISO 10993-18
  • ANSI/AAMI BE 83 2006

Test Options

Standard turn around times (TAT) are listed below. Ask an expert for a specific consultation on your product. If you are ready to submit your samples for testing, click here to fill out the Sample Submission Form.

Code Test TAT (days) Request Quote
IRX100 FTIR sample analysis, complete w/ library & peak labels, each 5 Add
IRX200 FTIR sample analysis (Microscopic), complete w/ library & peak labels, each 5 Add
IRX220 FTIR: (Microscopic) additional concurrent scans Add
IRX100 - FTIR sample analysis, complete w/ library & peak labels, each
TAT: 5 days
IRX200 - FTIR sample analysis (Microscopic), complete w/ library & peak labels, each
TAT: 5 days
IRX220 - FTIR: (Microscopic) additional concurrent scans
Standard TAT not available for this product

Sample Specifications

IRX100: 10 mg
IRX200: Minimum >20 µm

Study Outline

In the FTIR test procedure, spectra are compared to commercial or privately created libraries and the top matches with their percent comparison are reported.

An infrared spectrum is characteristic of a particular compound, providing information about its functional groups, molecular geometry and inter-/intra-molecular interactions. The infrared spectrometer provides a record of the infrared absorbency or transmittance of a sample as a function of wave number. The frequencies at which absorption occurs may indicate the type of functional groups present in the substance. The absence of an absorption band may indicate the absence of a functional group.

Electromagnetic radiation of characteristic frequencies interacts with electric dipoles within the molecule, resulting in absorption of radiation by the molecules. The characteristic frequencies for a particular molecule are determined by its vibrations, dependent upon the masses of the atoms of the molecule, their spatial geometry and the strengths of the connecting bonds.

Mathematical analysis of the raw data applies a Fourier Transformation (FT) and results in the spectrum showing characteristic frequencies in the infrared range between 4000 cm-1 and 400 cm-1.

A background spectrum is run using the same technique as the sample. The sample is run and the spectra printed. A library search can be run and the five best fits are printed.
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