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Category: Disinfectant Tests
Validation of sanitizing agents for effectiveness against organisms found in cleanroom environments.
[ more ]Tests available for Disinfectant Tests
Disinfectant kill time test
This test was designed to demonstrate log reduction values over time for a disinfectant against selected bacteria, fungi, and/or mold. The most common organisms tested include: Bacillus subtilis, Bacillus atrophaeus, Bacillus thuringiensis, Staphylococcus aureus, Salmonella cholerasuis, Pseudomonas aeruginosa, Aspergillus niger, and Trichophyton mentagrophytes.
Surface Disinfectant Efficacy Validation
Validation of sanitizing agents for effectiveness against organisms is increasingly an area of concern to manufacturers and regulatory agencies alike. Two ways to help validate a disinfectant program include testing the disinfectant by performing a time kill procedure of the use dilution in a liquid test matrix, or testing the kill rate after the disinfectant is applied to contaminated surfaces. This test procedure was designed to test the kill rate after a disinfectant is applied to contaminated coupons and is based on the AOAC Official Method 961.02 Germicidal Spray Products, with the exception that this is a quantitative test. Anywhere from three to ten replicates for each combination can be tested upon request.
General screening test, disinfection
This test meets FDA and EPA criteria for testing hard surface disinfectant efficacy against Staphylococcus aureus, Salmonella cholerasuis, and Pseudomonas aeruginosa dried onto stainless steel penicylinders. A 95% confidence level can be obtained by demonstrating kill of 59/60 replicates for each organism on three different lots of product.
Regimen test for contact lens ISO 14729
This test meets FDA 510(k) and ISO-14729 criteria for testing contact lens disinfectants according to a regimen of cleaning, rinsing, and soaking as recommended by the manufacturer. Lenses are challenged with Pseudomonas aeruginosa, Staphylococcus aureus, Serratia marcescens, Candida albicans, and Fusarium solani.
Antimicrobial finishes on fabrics AATCC method 100
Antimicrobial; parallel streak AATCC Method 147
Minimum inhibitory concentration (MIC)
The minimum inhibitory concentration (MIC) of an antibacterial is defined as the maximum dilution of the product that will still inhibit the growth of a test microorganism. The minimum lethal concentration (MLC) of an antibacterial is defined as the maximum dilution of the product that will kill a test organism. MIC/MLC values can be determined by a number of standard test procedures. The most commonly employed methods are the tube dilution method and agar dilution methods. Serial dilutions are made of the products in bacterial growth media. The test organisms are then added to the dilutions of the products, incubated, and scored for growth. This procedure is a standard assay for antimicrobials. The procedure incorporates the content and intent of the American Society for Microbiology (ASM) recommended methodology.
Minimum lethal concentration (MLC)
The minimum inhibitory concentration (MIC) of an antibacterial is defined as the maximum dilution of the product that will still inhibit the growth of a test microorganism. The minimum lethal concentration (MLC) of an antibacterial is defined as the maximum dilution of the product that will kill a test organism. MIC/MLC values can be determined by a number of standard test procedures. The most commonly employed methods are the tube dilution method and agar dilution methods. Serial dilutions are made of the products in bacterial growth media. The test organisms are then added to the dilutions of the products, incubated, and scored for growth. This procedure is a standard assay for antimicrobials. The procedure incorporates the content and intent of the American Society for Microbiology (ASM) recommended methodology.